Unknown bacteria lab report papers. Gram Negative Unknown Lab Report 2019-02-23

Unknown bacteria lab report papers Rating: 6,2/10 327 reviews

Microbiology Unknown Paper. Essay Example

unknown bacteria lab report papers

The plate had contamination, which prevented further testing from happening. Discussion This experiment required many different biochemical tests to be conducted for a few reasons; the first being to identify which bacteria was the one that was randomly selected and secondly for educational purposes so that students will become familiar and gain experience about the different types of media and laboratory techniques. Creamy 2 — darker off white, creamy appearance growth. The pattern of growth is bitted. In this experiment, researchers classified an unidentified bacterium based on its physical structure, colony morphology, optimal conditions and metabolic properties. The metabolic profile was created by inoculating the bacterium into broths containing glucose, lactose, mannitol, and citrate and incubating the tubes at 25° C for 48 hours, then observing it for color change. It is most commonly found in mammals intestines.

Next

Example of a Microbiology Unknown Lab Paper

unknown bacteria lab report papers

Sulfur is in the form of thiosulfate and there is also an iron-containing compound. A third test was done in order to confirm that Escherichia coli is the unknown. Both of the plates were labeled and stored in the refrigerator. It was performed for a third time and the result was negative. . To decipher between which biochemical tests to perform, the gram positive and negative tables handed out by the Professor, were referred to. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics.


Next

Unknown Bacteria Lab Report

unknown bacteria lab report papers

From previous biochemical tests done in the semester, Pseudomonas aeruginosa was already suspected because of the green pigment of the original streak plate. The only problem that was encountered appeared when trying to isolate the gram positive bacteria. This narrowed down the unknown to 5 possible answers: Escherichia coli, Klebesiella pneumoniae, Enterobacter aerogenes, Proteus vulgaris, and Pseudomonas aeruginesa. The Gram stain resulted in a Gram negative rod. The colonies were rough granular , translucent, and white brownish color with black granules. Unknown Bacteria Lab Report Introduction The purpose to this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us by our instructor.

Next

How to Write a Microbiology Unknown Lab Report

unknown bacteria lab report papers

Not all of these tests were performed on every culture, as some were used only for gram positive or gram negative bacteria. Only one-half of the plate was streaked, the inoculating loop was again and a small portion of the culture from the first quadrant was used to streak the second quadrant, repeating this step to inoculate the remaining quadrant. This is known as the excited state. The purpose of this lab was to identify two unknown bacteria cultures using various differential tests. Some other general rules that should be followed are: Microbial nomenclature: The name of the bacterium should written and spelled correctly.

Next

Unknown Bacteria Lab Report

unknown bacteria lab report papers

Centers for Disease Control and Prevention, 02 Apr. My sample number was 18. Gas production was also monitored, looking for fissures produced by production of gas during fermentation. The finding could have been incorrect because the clearing around the bacterium might not have been as clear for the tester to notice. {Colonies are large, beige or.

Next

Microbiology Unknown Paper. Essay Example

unknown bacteria lab report papers

It can even lead to death in rare cases. By going through the steps to identify the microorganism, students began to comprehend infectious disease. This was the only test necessary to determine the unknown gram negative bacteria in unknown stock 123. A negative casein test removed S. For this test, I was able to finalize the result of my unknown to be Proteus vulgaris. Bacteria, Enzyme, Growth medium 2145 Words 7 Pages Purpose: To identify an unknown bacterial specimen using basic laboratory technique and biochemical tests. Agar dishes are sterile covered petri dishes filled with a solid medium in them, the agar, and used to test, grow and culture bacteria.


Next

Microbiology Unknown Bacteria

unknown bacteria lab report papers

Firstly, all of the Gram negative unknown possibilities were rods. Surgical procedures, intravenous catheter insertions and some antibiotic treatments are common procedures that can introduce E. The sample collected was extracted from intestinal region. Each test performed, provided us with some key information about the unknown microbes in question and how the bacteria function. The prelab must be completed prior to the day of the experiment. They are in the soil, in our food, and on plants and animals. The first technique being used is the Gram stain.

Next

Microbiology Unknown Lab Report

unknown bacteria lab report papers

Rapid urease-positive bacteria will turn the broth pink within 24 hours. If bacteria have the enzyme citrate-permease they can transport molecules that will be converted to pyruvate within the cell. Lastly casein test was performed, test showed positive result for the break down of casein. Gram staining involves doing a simple smear, drying, and then heat fixing. Based on the Unknown Chart provided there were three Gram Positive Cocci possibilities: Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis. It resembles that light orange pink tint as well. The nitrate test was used to show if a bacterium would be able to reduce nitrate to nitrite or other nitrogenous compounds.

Next

Unknown Bacteria Lab Report Essay

unknown bacteria lab report papers

Everything must be sterilized and precautions must be taken to stop bacteria from the air getting in. Overall, Staphylococcus aureus is a very hit or miss bacteria, it can either be treated fairly easy or just be downright lethal. With a sterilized inoculating loop the colony was separately streak , inoculated and incubated. There are numerous small colonies. Each student chose their own unknown bacteria according to the number.

Next